The Bornholmer Straße border crossing was one of the border crossings between East Berlin and West Berlin between 1961 and 1990. The crossing was named after the street on which it is located, Bornholmer Straße ("Bornholm Street"), which in turn was named after the Danish Baltic Sea island of Bornholm. The actual border between East and West Berlin ran along railway lines which were crossed by the Bösebrücke.

The Bornholmer Straße border crossing played the historic role of being the first border crossing to be opened during the fall of the Berlin Wall on 9 November 1989.