Louisville WaterWorks Museum opened in the west wing of a renovated and restored interior of Pumping Station No. 1 on Zorn Avenue at 3005 River Road in Louisville, Kentucky overlooking the Ohio River. The building was constructed from 1858 until 1860 as part of Louisville's original water works. It was listed as a National Historic Landmark in 1971.

Renovations began in January 2013 and the museum opened on March 1, 2014.