Martinsburg is a city in and the county seat of Berkeley County, West Virginia, United States. The population was 18,773 at the 2020 census, making Martinsburg the largest city in the Eastern Panhandle of West Virginia and the seventh-largest city in the state. It is a principal city of the Hagerstown–Martinsburg metropolitan area extending into Maryland, which had 293,844 residents in 2020.

Martinsburg was established by an act of the Virginia General Assembly that was adopted in December 1778 during the American Revolutionary War. Founder Major General Adam Stephen named the gateway town to the Shenandoah Valley along Tuscarora Creek in honor of Colonel Thomas Bryan Martin, a nephew of Thomas Fairfax, 6th Lord Fairfax of Cameron.

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