Owslebury is a village and civil parish in the county of Hampshire, in the south of England approximately 5 miles (8.0 km) outside Winchester. It lies within the administrative district of the City of Winchester.
The village lies at the top of a hill, on the Roman road from Winchester to the south coast near Portchester. Marwell Zoological Park is towards the south of the parish. Owslebury lies on the Pilgrims' Trail from Winchester to Mont Saint-Michel and the Monarch's Way from Worcester to Shoreham-by-Sea.